High-Performance Raman Spectroscopic System Based on a Single Spectrograph, CCD, Notch Filters, and a Kr+ Laser Ranging from the Near-IR to Near-UV Regions

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We describe the performance of a high-throughput system for detecting Raman spectra excited in the range from 320 to 750 nm. The system utilizes notch filters to reject Rayleigh scattering, a single polychromator as the dispersive element, and photon detection via a charge-coupled device. The filters, mirrors, and grating are changed to maximize performance in each excitation region. The excitation source consists of a Kr+ ion laser. Good-quality Raman data are reported for rhodamine 6G at 0.1 mM in methanol with the use of 752.5-nm excitation, and 0.2 mM flavin adenine dinucleotide (FAD) in tris buffer with the use of 647.1-nm excitation. High-quality resonance Raman data for 0.1 mM N-acetylglycine ethyl dithio ester in 5% CH3CN/H2O are also reported with the use of 324.0-nm excitation.

Keywords: Near IR; Near UV; Raman system

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702934066145

Affiliations: 1: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A OR6, Canada 2: Kaiser Optical Systems Inc., P.O. Box 983, Ann Arbor, Michigan 48106, U.S.A.

Publication date: November 1, 1993

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