Skip to main content

New Instrumentation for Use in Excitation-Emission Fluorescence-Polarization Measurements

Buy Article:

$29.00 plus tax (Refund Policy)


A fluorescence excitation-emission matrix (EEM) is an excellent way to determine a specific substance in a multicomponent sample. In the present investigation, a novel, rapid-scanning spectrofluorometer has been developed for measuring an EEM; the instrument employs a 0.35-meter monochromator for choosing the excitation wavelength and a continuously variable interference filter for selecting the emission wavelength. With the system, a 51 × 93 excitation-emission matrix can be collected in 3.2 min, and detection limits of 500 ng/L are obtained for fluorescein dye in ethanol. Fluorescence polarization measurements in the plane parallel and perpendicular to the polarized excitation light adds another dimension to the instrument for characterizing a sample. The instrument is controlled by an IBM XT computer although data analysis is performed on a Macintosh computer with Spyglass software. A series of two-dimensional contour plots and multidimensional excitation-emission-polarization plots can be rapidly generated.

Keywords: Fluorescence; Fluorescence polarization; Instrumentation; Luminescence; Multidimensional fluorescence; Spectroscopic techniques

Document Type: Research Article


Affiliations: Department of Chemistry, Indiana University, Bloomington, Indiana 47405

Publication date: October 1, 1993

More about this publication?

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more