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Quantitative Analysis of Peptides by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

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Abstract:

Quantitative analysis of peptides using internal standards was carried out with the use of matrix-assisted laser desorption/ionization (MALDI) on a time-of-flight mass spectrometer in a ferulic acid matrix. Spectra were collected and averaged over several areas of the sample surface at constant laser energy, and a defocused beam was used to improve reproducibility. A calibration curve was plotted, showing a correlation coefficient of 0.992 and a relative standard deviation of 1.83%. The accuracy and precision of this technique were sensitive to the crystal structure of the matrix and saturation effects of the instrument's detector.

Keywords: LAMMA; Lasers; MALDI; Mass spectrometry; Peptides

Document Type: Rapid Communication

DOI: http://dx.doi.org/10.1366/0003702934067847

Affiliations: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

Publication date: August 1, 1993

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