Magic Angle Lifetime Measurements in Evanescent Wave Fluorometry

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

For fluorescence lifetime measurements in isotropic solutions, it is well known that detection using an emission polarization of 54.7°, the so-called magic angle, eliminates contribution of molecular reorientation to the fluorescence decay function. For macroscopically oriented samples and evanescent wave excitation, the magic angle may not necessarily be the 54.7°. With the use of a typical optical scheme of evanescent wave excitation and detection along the surface normal, a mathematical expression is derived for the magic angle condition. The magic excitation angle is shown to be a function of the angle of incidence of the excitation beam and the refractive indices of the two media involved in total internal reflection. The magic angle relations are valid regardless of the orientational distribution of molecules along the surface normal. The magic angle relations apply to cases where the in-plane orientational distribution is isotropic.

Keywords: Fluorescence; Spectroscopic techniques; Surface analysis; Time-resolved spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702934067298

Affiliations: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716

Publication date: May 1, 1993

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more