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Analysis of Urinary Calculi of Mixed and Unusual Composition: Raman Spectroscopic Investigation

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Raman spectroscopic analysis of kidney stones of mixed composition and of unusual stones is presented. Raman spectroscopy was used to analyze the chemical composition of kidney stones without extraction. A kidney stone sample was irradiated by laser light of 514.5 nm, and the scattered light was analyzed by a Raman spectrometer. The first stone was determined to be a mixture of calcium oxalate dihydrate and hydroxyapatite and the second one to be a mixture of calcium oxalate monohydrate, uric acid, and hydroxyapatite. The third and fourth stones were not mixed-composition stones, but rather stones with unusual composition. One was concluded to be carboxyapatite, and the other was mucoprotein-type stone. The present investigation indicates that Raman spectroscopy is a useful tool for direct analysis of kidney stone without going through the usual procedure of crushing, extracting, and carrying out tedious wet chemical analysis.

Keywords: Kidney stones; Raman spectroscopy; Urinary calculi

Document Type: Research Article


Affiliations: 1: Department of Biochemistry, Colorado State University, Fort Collins, Colorado 80523, U.S.A. 2: Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India 3: Institute of Surgery, East-Siberian Department, Siberian Division of Medical Science, Irkutsk, Russia

Publication date: March 1, 1993

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