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The application of charge-coupled device (CCD) technology to high-resolution luminescence microscopy is a recent development. The very high sensitivity, linear response, and field uniformity that are characteristic of a slow-scan, scientific CCD camera yield data of sufficient quality
to make quantitative image analysis possible. However, the low temporal resolution of current CCD cameras is a major disadvantage. A CCD with a read noise level of less than 10 electrons typically has a readout rate in the 40–200 kHz range, which precludes the acquisition of a time-resolved
image integrated over multiple excitation pulses without the use of an external gating device. Imaging in the time domain can potentially increase both the information content and the sensitivity of luminescence microscopy. For example: (1) fluorescent probe distributions can be resolved temporally
as well as spatially, yielding improved image contrast; and (2) temporally gated detection of long-lived luminescence discriminates against scattered light and autofluorescence, which can yield improved sensitivity.
Department of Chemistry, University of Arizona, Tucson, Arizona 85721
Publication date: February 1, 1993
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The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)