Provider: ingentaconnect
Database: ingentaconnect
Content: application/x-research-info-systems
TY - ABST
AU - Arakaki, Lorilee S. L.
AU - Burns, David H.
TI - Multispectral Analysis for Quantitative Measurements of Myoglobin Oxygen Fractional Saturation in the Presence of Hemoglobin Interference
JO - Applied Spectroscopy
PY - 1992-12-01T00:00:00///
VL - 46
IS - 12
SP - 1919
EP - 1928
KW - Chemometrics
KW - Absorption spectroscopy
KW - Oxygen
KW - Myoglobin
KW - UV-visible spectroscopy
N2 - Quantitative values for myoglobin oxygen fractional saturation were extracted from visible absorption spectra of myoglobin and hemoglobin solutions by analysis with three algorithms: classical least-squares, partial least-squares, and stagewise multiple linear regression. In an effort
to mimic *in vivo* conditions, oxygen tensions and concentrations of myoglobin and hemoglobin solutions in separate cuvettes were varied independently. Transmission measurements were made through both cuvettes so that spectra contained contributions from both myoglobin and hemoglobin.
Oxygen tensions in the myoglobin solutions spanned the rapidly varying region of the myoglobin oxygen saturation curve with pO_{2} ranging from 0 to 4.79 Torr, corresponding to fractional saturation values between 0 and 0.903. A range of hemoglobin oxygenations from fully oxygenated
to fully deoxygenated was used. Estimation of myoglobin fractional saturation by the classical least-squares algorithm had a standard error (SE_{est}) of 0.094, while the partial least-squares method resulted in an SE_{est} of 0.070. Partial least-squares estimations resulted
in an SE_{est} of 0.041 when a limited wavelength range was used. The stagewise multiple linear regression method had an SE_{est} of 0.052. Results indicate that stagewise regression and partial least-squares yielded estimates of myoglobin fractional saturation that were more
accurate than those obtained from classical least-squares.
UR - http://www.ingentaconnect.com/content/sas/sas/1992/00000046/00000012/art00027
M3 - doi:10.1366/0003702924123412
UR - http://dx.doi.org/10.1366/0003702924123412
ER -