Fluorometric Data Processing Strategies Using Nanosecond Waveform Digitizers

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Developments in commercially available digitization technology have made practical the software processing of nanosecond fluorometric data. The data are digitized and transferred to a microcomputer after each excitation pulse, then processed by a software module simulating either analog or photon counting detection. Software anode current processing provides display of the decay and integration of its area in the same operation, offers a multiplex advantage to improve decay collection rate, and allows the definition of a nearly rectangular gating function with sharp 1-ns edges. Application of the photon counting method to fluorometric instruments based on low-repetition-rate lasers is achieved with a multistop, multilevel discrimination algorithm to increase the usable detection rate. The ability to achieve photon counting detection permits a more rigorous statistical treatment of the data and can provide transit time-spread limited resolution.

Keywords: Fluorescence; Lasers, pulsed; Time-resolved spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702924123395

Affiliations: 1: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907; present address: National Center for Atmospheric Research, P.O. Box 3000, Boulder, CO 80303 2: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907

Publication date: December 1, 1992

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more