A Molecular Fluorescence Spectrometer that Corrects for the Effects of Quenching

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Abstract:

A fluorescence spectrometer has been constructed that simultaneously determines fluorescence intensity and excited-state lifetime. The ratio of intensity to lifetime is used to correct for the reduction in fluorescence intensity caused by collisional quenching. A frequency-domain technique is used to determine the excited-state lifetime in real-time. Computer-driven data acquisition provides instantaneous readout of the fluorescence intensity, the excited-state lifetime, and the ratio of these quantities. Correction for quenching by oxygen is demonstrated.

Keywords: Instrumentation; Molecular fluorescence spectroscopy; Spectroscopic techniques

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702924124330

Affiliations: 1: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824-1322; present address: Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada 2: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824-1322

Publication date: June 1, 1992

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