A Molecular Fluorescence Spectrometer that Corrects for the Effects of Quenching

$29.00 plus tax (Refund Policy)

Buy Article:


A fluorescence spectrometer has been constructed that simultaneously determines fluorescence intensity and excited-state lifetime. The ratio of intensity to lifetime is used to correct for the reduction in fluorescence intensity caused by collisional quenching. A frequency-domain technique is used to determine the excited-state lifetime in real-time. Computer-driven data acquisition provides instantaneous readout of the fluorescence intensity, the excited-state lifetime, and the ratio of these quantities. Correction for quenching by oxygen is demonstrated.

Keywords: Instrumentation; Molecular fluorescence spectroscopy; Spectroscopic techniques

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702924124330

Affiliations: 1: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824-1322; present address: Department of Chemistry, University of British Columbia, Vancouver, BC V6T 1Z1, Canada 2: Department of Chemistry, Michigan State University, East Lansing, Michigan 48824-1322

Publication date: June 1, 1992

More about this publication?
Related content



Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more