Determination of Effective Depth and Equivalent Pathlength for a Single-Fiber Fluorometric Sensor

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The relationship between sample depth and the corresponding fluorescence signal excited and collected with a single-fiber fluorometric optical sensor has been theoretically derived. The effective depth, zeff , is defined as the sample depth from which 90% of the total fluorescence signal originates. This parameter can be theoretically predicted as a function of the fiber radius, r 0, the acceptance angle, α, and the sample absorbance. For solutions of negligible absorbance, the effective depth is zeff =9r 0 tan −1α. The theoretical expressions have been experimentally tested with the use of 100- and 200-μm-core fibers and Rhodamine 6G solutions of different absorbances.

Keywords: Attenuation effects; Effective depth; Optical fluorometric sensors

Document Type: Research Article


Affiliations: Department of Chemistry, University of Louisville, Louisville, Kentucky 40292

Publication date: June 1, 1992

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