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Versatile and High-Repetition Subnanosecond Light Source for Fluorescence Lifetime Measurements

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Abstract:

We describe the construction and emission characteristics of a gap-adjustable capacitor-replaceable pulsed light source (air-discharge type) that produces high-repetition pulsed UV light of nanosecond duration. Stable light pulses of 0.8 to 3.5 ns width are generated from atmospheric-pressure air discharge between the sharp anode and round cathode. Appreciable heterogeneities on the distribution of emission intensity and its on-set timing along the discharge gap were observed. Fluorescence lifetimes of ethidium bromide solutions were measured to demonstrate the usefulness of the light source.

Keywords: Discharge; Fluorescence lifetime measurement; Instrumentation; Pulsed light source; Spectroscopic techniques; Time-resolved spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702924125159

Affiliations: 1: Department of Mechanical Engineering, Faculty of Engineering, University of Tokushima, Jousanjima, Tokushima 770, Japan 2: Department of Mechanical Engineering, Faculty of Engineering, University of Tokushima, Jousanjima, Tokushima 770, Japan; present address: Shimadzu Co. Ltd., Kyoto, Japan 3: Department of Applied Physics, Faculty of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565, Japan 4: Department of Applied Physics, Faculty of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565, Japan; present address: School of Medicine, Dept. of Cell Biology and Anatomy, University of North Carolina, Chapel Hill, NC, U.S.A.

Publication date: March 1, 1992

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