On the Feasibility of Passive Optical Separation of Scatter and Fluorescence

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Abstract:

The use of "flat" focusing devices, such as Fresnel lenses and holographic optical elements, for the passive optical separation of fluorescence and scattered light is evaluated theoretically and experimentally. Although "flat" lenses do not focus incident light isochronically, and should therefore have different spatial focusing characteristics for very short pulses (scattered light) and pseudo-continuous-wave signals (fluorescence), the optical quality of the flat lenses tested is insufficient to achieve such discrimination. Additionally, if the coherence length of the fluorescence is less than the difference in optical pathlength from extreme positions on the flat lens, fluorescence cannot be considered to be a pseudo-cw signal; this limitation constrains the potential applications of such instrumentation.

Keywords: Fresnel lenses; Holographic optical elements; Instrumentation, scattering; Instrumentation, time-resolved fluorescence; Passive optical time resolution

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702924125302

Affiliations: 1: Department of Chemistry, Indiana University, Bloomington, Indiana 47405; current address: Department of Chemistry, University of Massachusetts, Amherst, MA 01003 2: Department of Chemistry, Indiana University, Bloomington, Indiana 47405; permanent address: Unilever Research, Port Sunlight Laboratory, Quarry Road East, Bebington, Wirral, Merseyside L63 3JW, United Kingdom 3: Department of Chemistry, Indiana University, Bloomington, Indiana 47405; permanent address: CLS-2, MS G738, Los Alamos National Laboratory, Los Alamos, NM 87545 4: Department of Chemistry, Indiana University, Bloomington, Indiana 47405

Publication date: March 1, 1992

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