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Axial-Beam Absorption Detection for Capillary Electrophoresis with a Conventional Light Source

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Abstract:

A commercial absorption detector was modified to provide axial-beam absorption detection for capillary electrophoresis. The increased path length led to improved detection limits. By the use of a nonaqueous solvent, the column is made into an optical waveguide for highly efficient light transmission. The electropherogram integrates the absorbance produced by all components in the sample, but provides information identical to that from normal electropherograms. The mechanical modifications make it possible to switch readily between the cross-beam and the axial-beam geometries.

Keywords: Instrumentation; Lasers; Optics; Spectroscopic techniques

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702914335931

Affiliations: Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011

Publication date: August 1, 1991

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