Authors: Yamamoto, H.¹; Yoshikawa, O.²; Nakatani, M.²; Tsuji, F.²; Maeda, T.²

Source: Applied Spectroscopy, Volume 45, Issue 7, Pages 1067-1207 (August 1991) , pp. 1166-1170(5)

Publisher: Society for Applied Spectroscopy

Abstract:

Direct measurement by Fourier transform near-infrared (FT-NIR) spectrometry on thin-layer chromatographic plates is described. Zones separated on a high-performance thin-layer chromatographic plate fixed on the sample stage of a microscope linked with an FT-NIR spectrometer were scanned with a narrow beam of light (0.4-mm square). The NIR chromatogram and spectra of the separated zones were then obtained by the diffuse transmission method. Three kinds of phospholipids that have essentially no absorption bands in the ultraviolet and visible regions but that do have native absorption bands in the NIR region were used to evaluate the usefulness of this method. The limit of detection for phosphatidylcholine was under 1 μg, and the correlation coefficient of the calibration curve obtained from derivative spectra was 0.998 for amounts from 1.25 to 10 μg.

Keywords: Fourier transform near-infrared spectrometry; High-performance thin-layer chromatography; Diffuse transmission; Derivative spectra; Microspectrometry

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702914335968

Affiliations: 1: Shimadzu Corporation, Nakagyo-ku, Kyoto 604, Japan; complete address: R&D Engineering Department, Analytical Instrument Division, Shimadzu Corporation, 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto 604, Japan 2: Shimadzu Corporation, Nakagyo-ku, Kyoto 604, Japan

Publication date: 1991-08-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Yamamoto, H. ;  Yoshikawa, O. ;  Nakatani, M. ;  Tsuji, F. ;  Maeda, T.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers