Three-Dimensional Space- and Time-Resolved Fluorescence Spectroscopy

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Abstract:

A fluorescence spectroscopic system with both picosecond time resolution and submicrometer three-dimensional space resolution has been developed for elucidating the dynamics of inhomogeneous photophysical/photochemical phenomena. This system is based on a confocal laser scanning fluorescence microscope and a time-correlated single photon counting method. Results on demonstration experiments are shown to specify the performance of the system. Characteristics and limitations of the system are discussed.

Keywords: Fluorescence microscope; Laser spectroscopy; Three-dimensional resolution; Time-resolved spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702914336336

Affiliations: 1: Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Kyoto Research Park, Chudoji-minami, Shimogyo-ku, Kyoto 600, Japan 2: Microphotoconversion Project, ERATO, Research Development Corporation of Japan, Kyoto Research Park, Chudoji-minami, Shimogyo-ku, Kyoto 600, Japan; permanent address: Department of Applied Physics, Osaka University, Suita, Osaka 565, Japan

Publication date: July 1, 1991

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