@article {Remsen:1991:0003-7028:868,
	author = "Remsen, Edward E. and Freeman, John J.",
	title = "A Size-Exclusion Chromatography/FT-IR (SEC/FT-IR) Technique for Improved FTIR Spectroscopy of Proteins in D2O Solution",
	journal = "Applied Spectroscopy",
	volume = "45",
	number = "5",
	year = "1991",
	abstract = "A size-exclusion chromatography/Fourier transform infrared spectroscopic (SEC/FT-IR) method for improving the FT-IR spectroscopy of proteins in D<SUB>2</SUB>O solution is described. The technique employs low-volume SEC columns to exchange protein solutions into buffered D<SUB>2</SUB>O and effectively removes spectral interference from H<SUB>2</SUB>O and other low-molecular-weight compounds in the region of the protein amide carbonyl stretching band (1600-1700 cm<SUP>&#8722;1</SUP>). Application of the method with protein solutions prepared in deuterated, denaturing buffers completely exchanges labile protein hydrogens with deuterium and refolds the proteins into their native conformations for subsequent spectral characterization. Potential applications of the SEC/FT-IR method for kinetic monitoring of dynamic processes in proteins (e.g., secondary structure formation during protein folding) are also discussed.",
	pages = "868-873",
	url = "http://www.ingentaconnect.com/content/sas/sas/1991/00000045/00000005/art00021",
	doi = "doi:10.1366/0003702914336543",
	keyword = "Size-exclusion chromatography, Fourier transform infrared spectroscopy, FT-IR spectroscopy of proteins in D2O"
}