Laser-Induced Fluorescence Detection of Rhodamine-6G at 6 × 10−15 M

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Abstract:

We have demonstrated sensitive fluorescence detection of rhodamine-6G in aqueous solutions down to ∼10−15 M with a detection limit of 6 × 10−15 M. The working curve was observed to be linear over four orders of magnitude and could be extended to higher concentrations. Single molecules of rhodamine-6G had a transit time of 1.8 ms through the 11 pL, probe volume. At 2 × 10−14 M, the probability of a molecule being in the 11 pL probe volume is ∼0.1. Analysis of the data indicates that the signal-to-noise ratio for the detection of a single molecule of rhodamine-6G is ∼1.

Keywords: Fluorescence; High sensitivity; Laser

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702914336453

Affiliations: 1: Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545; present address: Department of Chemistry, Pohang Institute of Science and Technology, P.O. Box 125, Pohang, 790-600, Republic of Korea 2: Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545

Publication date: June 1, 1991

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