Authors: Eckert-Tilotta, S.E.¹; Scouten, W.H.²; Hines, John³

Source: Applied Spectroscopy, Volume 45, Issue 3, Pages 325-515 (March/April 1991) , pp. 491-495(5)

Publisher: Society for Applied Spectroscopy

Abstract:

Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose. An optrode is constructed by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable. Because fluorescence is enhanced as Ca⁺² binds with the immobilized dansylated troponin (TnC_DANZ), this system can be used as a calcium sensor for monitoring plasma Ca⁺² levels.

Keywords: Biosensors; Fiber optics; Fluorescence spectroscopy; Dansylaziridine; Troponin; Calcium determination; Optrodes

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702914337245

Affiliations: 1: Department of Chemistry, Baylor University, Waco, Texas 76798; present address: Department of Chemistry, University of North Dakota, Grand Forks, ND 58202 2: Department of Chemistry, Baylor University, Waco, Texas 76798 3: Department of Chemistry, Baylor University, Waco, Texas 76798; address: NASA/Ames Research Center, Moffett Field, CA 94035

Publication date: 1991-03-01

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