A Calcium-Selective Optrode Based on the Fluorescence of Dansylated Troponin

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Abstract:

Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose. An optrode is constructed by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable. Because fluorescence is enhanced as Ca+2 binds with the immobilized dansylated troponin (TnCDANZ), this system can be used as a calcium sensor for monitoring plasma Ca+2 levels.

Keywords: Biosensors; Calcium determination; Dansylaziridine; Fiber optics; Fluorescence spectroscopy; Optrodes; Troponin

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702914337245

Affiliations: 1: Department of Chemistry, Baylor University, Waco, Texas 76798; present address: Department of Chemistry, University of North Dakota, Grand Forks, ND 58202 2: Department of Chemistry, Baylor University, Waco, Texas 76798 3: Department of Chemistry, Baylor University, Waco, Texas 76798; address: NASA/Ames Research Center, Moffett Field, CA 94035

Publication date: March 1, 1991

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