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Quantitative Treatment of UV Resonance Raman Spectra of Biological Molecules: Application to the Study of Membrane-Bound Proteins

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A set of instrumental and digital signal processing techniques (Fourier self-deconvolution and the maximum entropy method) was adopted to detect and treat low-signal-level UV resonance Raman spectra of biological molecules and supramolecular complexes. The applicability of the methods for analysis of ATP binding in the active site of Na+,K+-ATPase, as well as for investigation of aromatic amino acids involved in the functioning of bacterial and visual rhodopsins, is demonstrated.

Keywords: Digital signal processing; Low-level Raman signal; Membrane-bound proteins; Ultraviolet resonance Raman spectroscopy

Document Type: Research Article


Affiliations: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Ul. Miklukho-Maklaya, 16/10, 117871 GSP Moscow, V-437 USSR

Publication date: February 1, 1991

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