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A Correction Method for Fluorescence Reduction Using Time-Resolved Fluorometry and Spectrophotometry

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A method to correct fluorescence reduction due to foreign materials and temperature using time-resolved fluorometry and spectrophotometry has been developed. The factors taken into consideration were dynamic quenching and inner filter effects on the excitation beam and the fluorescence, and the fluorescence intensity with respect to these factors was theoretically analyzed. A theoretical approach for correcting the fluorescence intensity was obtained, with the use of the fluorescence lifetime, the absorbance at the excitation wavelength, and the absorbance at the fluorescence wavelength. Since these parameters are observable, a novel method can be applied to samples in cases where foreign materials and temperature are unknown. The proposed correction method is also suitable for noncontact and remote measurements. From experiments using a UO2 2+ ion, the proposed method was verified.

Keywords: Absorbance; Dynamic quenching; Fluorescence lifetime; Fluorometry; Inner filter effect

Document Type: Research Article


Affiliations: 1: Energy Research Laboratory, Hitachi, Ltd., 1168 Moriyama, Hitachi, Ibaraki 316, Japan 2: Energy Research Laboratory, Hitachi, Ltd., 1168 Moriyama, Hitachi, Ibaraki 316, Japan; Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo 113, Tokyo

Publication date: January 1, 1991

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