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Preparation of a Tissue Model for Quantitative FT-IR Microspectroscopic Functional Group Imaging

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Abstract:

There is much interest in the biological and pharmacological sciences in knowing the specific location, concentration, and chemical structure of biologically active molecules in tissue. Infrared spectroscopy can be a suitable method for addressing these considerations; it can give quantitative and qualitative information without destroying the sample. IR spectra of approximately 20-μm-thick slices of animal tissue, mounted on barium or calcium fluoride crystals, can be obtained in the transmittance mode. Also, 3–5-μm-thick tissue slices can be mounted on gold-coated glass slides, and IR spectra can be obtained in the reflection-absorption mode.

Keywords: Analytical methods; Infrared

Document Type: Short Communication

DOI: http://dx.doi.org/10.1366/0003702904417841

Affiliations: Bristol-Myers Pharmaceutical Research and Development Division, P.O. Box 5100, Wallingford, Connecticut 06492-7660

Publication date: November 1, 1990

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