Authors: Vessières, Anne¹; Jaouen, Gerard¹; Salmain, Michele¹; Butler, Ian S.²

Source: Applied Spectroscopy, Volume 44, Issue 6, Pages 911-1096 (July 1990) , pp. 1092-1094(3)

Publisher: Society for Applied Spectroscopy

Abstract:

During some recent work on the application of organometallic IR markers in the detection of hormonal steroid receptors and the determination of immunological doses, we needed to measure quantitatively amounts in the 10-picomole range in solution. Previously, we had obtained data for the solid state by using 3.0-mm micro-pellets of the precipitated proteins by themselves or when diluted in KBr. The detection limit in both cases was about 80 picomoles. For solution work, there are micro-cavity cells commercially available which have pathlengths of 1.0 mm and working volumes of about 10 μL. These cells proved unsuitable for our purposes, however, since we needed to detect amounts of organometallic marker as low as 0.1 picomole.

Keywords: Infrared

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702904086894

Affiliations: 1: Ecole Nationale Superieure de Chimie de Paris, 11 rue Pierre et Marie Curie, 75231 Paris, Cedex 05, France 2: Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montreal, Quebec, Canada H3A 2K6

Publication date: 1990-07-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Vessières, Anne ;  Jaouen, Gerard ;  Salmain, Michele ;  Butler, Ian S.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers