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Time-Resolved Fluorescence Microscopy Using Multichannel Photon Counting

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Abstract:

A new measurement system is reported which combines fluorescence microscopy with multichannel photon-counting detection. The prototype instrument can be operated in normal fluorescence and in fluorescence lifetime modes. Multichannel photon detection was used in order to enhance the rate of data collection. The feasibility of rapid two-dimensional mapping of samples was indicated. The technique may prove especially valuable in situations where multicomponent samples display similar spectral features but differing lifetime characteristics. By increases in the measurement dimensionality, enhanced sample evaluation is possible. Applications involving model and practical biological systems are discussed.

Keywords: Fluorescence lifetime measurements; Fluorescence microscopy; Multichannel photon counting; Spectroscopic techniques

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702904085868

Affiliations: 1: Department of Applied Physics, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan 2: Department of Applied Physics, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan; permanent address: Central Research Laboratory, Hitachi Ltd. Kokubunji, Tokyo 185, Japan 3: Department of Applied Physics, Faculty of Engineering, Osaka University, Suita, Osaka 565, Japan; permanent address: Wayne State University, Department of Chemistry, Detroit, MI 48202, U.S.A.

Publication date: January 1, 1990

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