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Pointing-Stability-Limited Dual-Beam Absorbance Measurements by Position-Sensitive Detection

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A HeCd laser was examined as a light source for dual-beam absorbance measurements by position-sensitive detection. The measurement was limited by the laser point stability and, also, unspecified laser radiation that was effectively reduced by a spatial and spectral filter combination. An absorbance detection limit of 5 × 10−4 AU (3 × rms baseline noise) was predicted from an experimental optimization procedure. The detection limit was supported by microbore high-performance liquid chronatography of a B-2 vitamin sample, detecting riboflavin absorbance at 325 nm. Issues regarding the improvement of dual-beam absorbance measurements by position-sensitive detection are addressed.

Keywords: Instrumentation, position-sensitive detection; Lasers, helium cadmium; Optics; UV-visible spectroscopy

Document Type: Research Article


Affiliations: Center for Process Analytical Chemistry, Department of Chemistry, BG-10, University of Washington, Seattle, Washington 98195

Publication date: November 1, 1989

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