High-Precision Fluorimetry with a Light-Emitting Diode Source
Abstract:A simple fluorimeter which uses a light-emitting diode as a source of excitation and a photodiode as a detector has been constructed and evaluated. The exceptional stability of both the detector and the source make it possible to make concentration measurements by fluorimetry with a precision of at least 0.002%. Furthermore, the system exhibited excellent linear dynamic range and a limit of detection of 20 ng/L for the dye Oxazine 720 in methanol.
Document Type: Research Article
Affiliations: Department of Chemistry, University of Florida, Gainesville, Florida 32611
Publication date: November 1, 1988
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