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High-Precision Fluorimetry with a Light-Emitting Diode Source

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Abstract:

A simple fluorimeter which uses a light-emitting diode as a source of excitation and a photodiode as a detector has been constructed and evaluated. The exceptional stability of both the detector and the source make it possible to make concentration measurements by fluorimetry with a precision of at least 0.002%. Furthermore, the system exhibited excellent linear dynamic range and a limit of detection of 20 ng/L for the dye Oxazine 720 in methanol.

Keywords: Fluorimetry, high precision; Light-emitting diode

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702884429535

Affiliations: Department of Chemistry, University of Florida, Gainesville, Florida 32611

Publication date: November 1, 1988

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