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MAGIC-LC/FT-IR Spectrometry: Preliminary Studies

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A new solvent elimination interface based on the Monodisperse Aersol Generation Interface for Combining Liquid Chromatography with Fourier transform infrared (MAGIC-LC/FT-IR) spectrometry is described. The solvent elimination efficiency of MAGIC-LC/FT-IR was studied by varying the mobile-phase composition from 100% methanol to 100% water. As the mobile-phase composition was varied, erythrosin B was injected into the interface and deposited on a KBr window after the solvent removal. Spectra were obtained which compared favorably with reference spectra, even as the mobile-phase water content was increased. A reverse-phase separation was completed to demonstrate that readily identifiable spectra can be obtained from mobile phases containing high percentages of water, without heating of the effluent stream.

Keywords: FT-IR chromatography; LC solvent elimination

Document Type: Research Article


Affiliations: 1: Department of Chemistry, School of Chemical Sciences, University of Georgia, Athens, Georgia 30602 2: School of Chemistry, Georgia Institute of Technology, Atlanta, Georgia 30332

Publication date: November 1, 1988

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