Authors: Hofstraat, J.W.¹; Gooijer, C.²; Velthorst, N.H.²

Source: Applied Spectroscopy, Volume 42, Issue 4, Pages 537-707 (May/June 1988) , pp. 614-619(6)

Publisher: Society for Applied Spectroscopy

Abstract:

Under certain conditions, highly specific fluorescence spectra, showing molecular vibrations as in infrared spectroscopy, can be obtained for the analysis of compounds separated in TLC and microbore LC. Two techniques, both employing low-temperature solid samples, are available: Shpol'skii spectroscopy and Fluorescence Line-Narrowing (FLN) spectroscopy. The former utilizes n-alkane solvents, which form regular, crystalline solids; the latter is based on selective laser excitation. Both techniques can be applied for detection in TLC. FLN is more feasible, since the Shpol'skii method requires spraying of the TLC plate, which leads to significant spreading of the spots. FLN can also be used for off-line detection in micro-LC after immobilization of the column effluent on a TLC plate. In addition, some exploratory experiments on the applicability of FLN for on-column detection in LC are presented.

Keywords: Fluorescence line-narrowing spectroscopy; Shpol'skii spectroscopy; Thin-layer chromatography; Liquid chromatography

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702884429229

Affiliations: 1: Department of General and Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, the Netherlands; present address: Ministry of Traffic and Public Works, Department of Public Works, Tidal Waters Division, Nijverheidsstraat 2, 2: Department of General and Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HV Amsterdam, the Netherlands

Publication date: 1988-05-01

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