Skip to main content

Aqueous Infrared Pharmaceutical Analysis of Two Choline Compounds by Flow Injection Analysis Using the CIRCLE Cell

Buy Article:

$29.00 plus tax (Refund Policy)


The advent of the cylindrical internal reflectance (CIRCLE) cell has solved the difficult problem of quantitation of solutes in aqueous solutions by infrared spectroscopy. By a combination of flow injection analysis (FIA) and Fourier transform infrared (FT-IR) spectroscopy with the CIRCLE cell, a fast, reproducible technique suited for the determination of two choline compounds of pharmaceutical interest—succinylcholine chloride and bethanechol chloride—was developed. Quantitation was carried out with the use of either the ester band at 1075 cm−1 for bethanechol or the trimethyl quaternary ammonium band at 953 cm−1 for succinylcholine. Linearity of standard solutions was achieved from about 0.5 to at least 50 ppt for both compounds, and detection limits of 0.02% w/v were possible. A sample throughput of 60 samples/h was estimated. Analysis of pharmaceutical preparations was performed after minor sample clean-up, which was necessary only in the case of the bethanechol chloride tablets.

Keywords: CIRCLE cell; Choline compounds; Flow injection analysis; Quantitative IR

Document Type: Research Article


Affiliations: 1: Department of Chemistry, Miami University, Oxford, Ohio 45056; present address: Department of Chemistry, Eastern Illinois University, Charleston, IL 61920 2: Department of Chemistry, Miami University, Oxford, Ohio 45056

Publication date: March 1, 1988

More about this publication?

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more