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Application of Legendre Polynomials to the Elimination of Baseline Variation in Biological FT-IR Spectra

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Abstract:

A method for eliminating baseline variation is proposed for use on biological FT-IR spectra. This method is motivated by the fact that baselines are often assumed to be low-degree polynomials. Spectra are expanded in terms of a set of orthonormal polynomials derived from the Legendre polynomials, and leading terms of the expansion, which contain most of the baseline variation, are removed. An application of this method to protein spectra is presented and discussed.

Keywords: Baseline correction; Computer, applications; Infrared spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702884427988

Affiliations: National Center for Biomedical Infrared Spectroscopy, Battelle Columbus Division, 505 King Avenue, Columbus, Ohio 43201

Publication date: March 1, 1988

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