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Attenuated total reflectance Fourier transform infrared (ATR/FT-IR) spectroscopy is a powerful and widely used technique for obtaining molecular structural information from thin films of material. The ability of the method to provide orientational information from partially ordered
samples such as lipid multibilayers (possibly with incorporated peptides) has resulted in several recent applications of the method to biophysical problems. In most ATR studies, the material under investigation is deposited as a dry film on an appropriate crystal, Ge or ZnSe being widely used
for this purpose. Obviously, the biological relevance of dry films is questionable, as water often forms an integral part of the in vivo macromolecular assembly. It is evidently desirable to study biological samples under conditions of full hydration, where the effects of variation
in pH and ionic strength and composition can be explored. Commercial ATR cells with water jackets are available, but these are expensive and fragile and have fairly large jacket volumes.
Department of Chemistry, Rutgers University, 73 Warren Street, Newark, New Jersey 07102
Publication date: January 1, 1988
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The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)