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A UV-VIS Spectrophotometer for Absorbance Measurements of Weakly Transparent Light Scattering Samples

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Absorption measurements by transmission through weakly transparent media present some difficulties with conventional commercial spectrophotometers when both good sensitivity and high resolution are required. We describe a home-made computerized spectrophotometer which has been designed for absorption measurements through poorly transparent highly light scattering solutions. Emphasis is given to the difficulties inherent in this type of measurement. Examples are presented to illustrate the effects of the sample thickness and of the fluorescence, which can substantially affect the measured absorbance.

Keywords: Computer, applications; Instrumentation, UV-visible spectroscopy; UV-visible spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702874447734

Affiliations: Photophysique Photochimie Moléculaire, UA CNRS 348, Université de Bordeaux I, F 33405 Talence, France

Publication date: September 1, 1987

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