Helium Neon Laser Intracavity Photothermal Beam Deflection Densitometer

Author: Tran, Chieu D.

Source: Applied Spectroscopy, Volume 41, Issue 3, Pages 349-531 (March/April 1987) , pp. 512-516(5)

Publisher: Society for Applied Spectroscopy

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Abstract:

A novel, compact He-Ne laser intracavity photothermal beam deflection densitometer has been developed. In this instrument, a TLC plate is placed inside the cavity of the He-Ne laser, which serves as a probe. An argon-ion pump-laser overlaps with the probe beam at the chromatogram spot. The apparatus is 34 times more sensitive than the corresponding extracavity instrument. The technique not only was able to detect bilirubin, bilirubin ditauride, and half-bilirubin on a developed HPTLC but also provided insight into the photophysical processes of those pigments. The detection limit for bilirubin, excited by the 476-nm laser line, at 20 mW intensity, modulated at 7 Hz, is 2.5 ng.

Keywords: Bilirubin; Intracavity; Photophysics; Photothermal deflection

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702874448850

Affiliations: Department of Chemistry, Marquette University, Milwaukee, Wisconsin 53233

Publication date: March 1, 1987

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