Diode Array Spectrophotometry of Translucent Materials

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The light scattered from translucent samples is used to obtain absorption-type spectra and provide quantitation of an absorber within the scattering bodies. Synthetic vesicles with an encapsulated dye, bromo-thymol blue (BTB), are employed as a novel model system to study the properties of translucent samples. Chlorophyll measurement in intact phytoplankton and algae is demonstrated. A least-squares fitting procedure is used to overcome the effect of chlorophyll fluorescence on the scattering data. A straight line relationship between a defined pseudo-absorbance and concentration is shown for both the BTB and chlorophyll data. A limit of detection of about 0.2 mg/L BTB is obtained for the synthetic vesicles. For chlorophyll the limit of detection is in the range of 1 to 5% of that found in the native plankton and algae samples.

Keywords: Chlorophyll; Diode Array; In vivo absorption spectra; Plankton; Scattering; Synthetic vesicles

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702864507774

Affiliations: Department of Chemistry, Florida State University, Tallahassee, Florida 32306

Publication date: November 1, 1986

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