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The Rapid Identification of Bacteria Using Time-Resolved Fluorescence and Fluorescence Excitation Spectral Methods

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Time-resolved fluorescence spectra have been obtained for Escherichia coli, Pseudomonas fluorescens, Staphylococcus epidermidis, and Enterobacter cloacae. Pseudomonas fluorescens has been shown to have distinctly different time-resolved spectra. Fluorescence excitation spectra for the three other organisms showing similar time-resolved spectra are very nearly alike, while spectra of Pseudomonas fluorescens are markedly different. This suggests that the changes in the average fluorescence lifetime with emission wavelength are due to the separate contributions from fluorophores of distinctly different lifetimes which have emission maxima at different wavelengths.

Keywords: Fluorescence; Time-resolved spectroscopy

Document Type: Research Article


Affiliations: 1: Department of Chemistry, University of Rhode Island, Kingston, Rhode Island 02881; on leave from the Chemistry Department, Asutosh College, Calcutta, India 2: Department of Chemistry, University of Rhode Island, Kingston, Rhode Island 02881 3: Department of Microbiology, University of Rhode Island, Kingston, Rhode Island 02881

Publication date: September 1, 1985

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