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Infrared Spectrophotometric Determination of 13C Enrichment

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A study has been made of the feasibility of using the v 3 band of 13CO2 as a basis for measurement of the extent of isotope enrichment in breath tests. Correlation and least-squares treatments were used. Considering the constraints of a small computer, the correlation approach is the more satisfactory. The results indicate that a method that is based on a single measurement at 170 Torr, with a standard curve for quantitation, will, give results that are adequate, considering the natural variation that occurs. Assuming the use of a beam condenser, the amount of sample required is less than that normally taken for this application.

Keywords: 13CO2 determination; Infrared spectroscopy; Isotope enrichment

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702844555007

Affiliations: Department of Chemistry, Florida State University, Tallahassee, Florida 32306

Publication date: September 1, 1984

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