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Rapid Scanning Constant Energy Synchronous Fluorescence Spectrometry: A Comparison of Flow Cells

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Abstract:

Rapid scanning constant energy synchronous fluorescence spectrometry (RSCESFS) is evaluated for flow cell design, scan speed, and constant energy interval choice. Analytical figures of merit for several polycyclic aromatic hydrocarbons (PAHs) are given for several experimental conditions, for several flow cells, and for both RSCESFS and conventional (nonscanning) fluorescence spectrometry. With RSCESFS, absolute detection limits as low as 700 fg, linear dynamic ranges as great as five orders of magnitude and the time to record a complete 350 nm spectrum as short as 17 s have been obtained.

Keywords: Flow cells; Fluorescence; Synchronous fluorescence

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702844554080

Affiliations: 1: Department of Chemistry, University of Florida, Gainesville, Florida 32611 2: Department of Chemistry, University of Florida, Gainesville, Florida 32611; present address: Eli Lilly & Co., Dept. of Analytical Develop., Indianapolis, IN 46285.

Publication date: March 1, 1984

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