Optical Fiber Fluoroprobes for Biological Measurements
Abstract:An optical fiber fluoroprobe has been designed to make in vivo fluorescence measurements in minute volumes of body fluids. The fluoroprobe is evaluated for conventional laser excited, two-photon excited, and sequentially excited modes of fluorescence detection. Limits of detection, approximately 5 × 10−7 M, were determined for test compounds measured in various solvents including cerebral spinal fluid and whole blood, and are comparable for the three fluorescence techniques. Linear dynamic ranges are shown to be solvent dependent and superior for the nonlinear excited fluorescence techniques. The ultimate detectability and selectivity of this technique are also discussed.
Document Type: Research Article
Affiliations: Department of Chemistry, University of Tennessee, Knoxville, Tennessee 37996-1600
Publication date: January 1, 1984
More about this publication?
- The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Request copyrighted SAS materials
- Spectroscopic Nomenclature
- Focal Point (Open Access)
- ingentaconnect is not responsible for the content or availability of external websites