A Simple Low-temperature Cryostat for Resonance Raman Studies of Frozen Protein Solutions

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

A frequent problem encountered in resonance Raman studies of aqueous protein solutions is that of protein denaturation due to localized heating by the laser beam. The problem has been circumvented, with varying degrees of success, by using spinning cells, flowing solutions through capillary tubes (both 90° scattering geometry), or by backscattering from cooled, spinning NMR tubes (135° scattering geometry). In our experience the latter technique is most successful. However, it has limitations when used to investigate the low-frequency region (0 to 500 cm−1), because broad nonresonant Raman scattering from the glass or quartz NMR tubes produces an ill-defined envelope of bands between 300 and 500 cm−1. These bands often dominate resonance Raman spectra in this region, obscuring weak sample bands (e.g., see Fig. 2). These difficulties have led us to design a simple cryostat that can be used to obtain resonance Raman spectra via 135° backscattering directly from the surface of a frozen protein solution (Fig. 1.)

Keywords: Cryostat; Iron-sulfur; Low temperature; Protein solution; Resonance Raman spectroscopy

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702834634550

Affiliations: Department of Chemistry, Princeton University, Princeton, New Jersey 08540; and College of Chemistry and Physics, Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804

Publication date: May 1, 1983

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more