Authors: Meiri, Zvi; Berezin, Yaakov; Shemesh, Avraham; Ehrenberg, Benjamin

Source: Applied Spectroscopy, Volume 37, Issue 2, Pages 101-210 (March/April 1983) , pp. 203-207(5)

Publisher: Society for Applied Spectroscopy

Abstract:

The interfacing of two spectrophotometric systems to an Apple II microcomputer is described. The first is a flash photometric setup in which light flashes are formed either with a chopper wheel, or with an acousto-optic modulator which is activated by the microcomputer. Spectroscopic transients are monitored with a time resolution as short as 250 ns, by counting photon pulses into the computer's memory in the fast direct memory access mode. The formation half-times of the M₄₁₂ intermediate in the photocycle of bacteriorhodopsin were measured in H₂O and in D₂O using the characteristic Raman band of this species, and are 68 ± 2 and 300 ± 5 μs, respectively. A microcomputer-controlled Raman spectrometer, in which the Apple II is interfaced to a Spex monochromator and the photon counting electronics, is also described. These microcomputer-controlled configurations provide simple and inexpensive setups for data collection in flash photometry and Raman and fluorescence spectroscopy.

Keywords: Microcomputer-controlled flash photometry; Microcomputer controlled Raman spectroscopy; Photon counting; Acousto-optic modulation; Bacteriorhodopsin kinetics

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702834634082

Affiliations: 1: Department of Physics, Bar-Ilan University, Ramat-Gan 52100, Israel

Publication date: 1983-03-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Meiri, Zvi ;  Berezin, Yaakov ;  Shemesh, Avraham ;  Ehrenberg, Benjamin

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers