Authors: Winters, S.¹; Gendreau, R.M.²; Leininger, R.I.²; Jakobsen, R.J.²

Source: Applied Spectroscopy, Volume 36, Issue 4, Pages 339-473 (July/August 1982) , pp. 404-409(6)

Publisher: Society for Applied Spectroscopy

Abstract:

The adsorption of proteins from ex vivo flowing sheep blood has been studied using FT-IR/ATR with spectra being collected at 0.8-s intervals of blood flow. We have found that protein adsorption can be divided into four adsorption regions: the first region being one of rapid adsorption of protein species such as albumin, an unknown protein species, glycoproteins, and some fibrinogen; in the second region, there is little increase in the total protein adsorbed, but there is evidence of protein replacement. Fibrinogen adsorption is the major event occurring in the third adsorption region, whereas the appearance of red blood cells and platelets marks the fourth region. Such knowledge of protein composition at various times in the adsorption process is providing molecular level information on thrombosis occurring on surfaces.

Keywords: FT-IR; Protein absorption; Blood-surface interactions; Biocompatibility

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702824639592

Affiliations: 1: Biological Spectroscopy Facility, Battelle-Columbus Laboratories, Columbus, Ohio 43201; present address: Department of Bioengineering, Merrill Hall, University of Utah, Salt Lake City, UT 84112 2: Biological Spectroscopy Facility, Battelle-Columbus, Laboratories, Columbus, Ohio 43201

Publication date: 1982-07-01

More about this publication?

• The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge.
  
  Previously published as Bulletin (Society for Applied Spectroscopy)
• Editorial Board
• Information for Authors
• Submit a Paper
• Subscribe to this Title
• Membership Information
• Request copyrighted SAS materials
• Spectroscopic Nomenclature
• Focus Compendium
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Analytical Chemistry
• By this author: Winters, S. ;  Gendreau, R.M. ;  Leininger, R.I. ;  Jakobsen, R.J.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers