Computer-controlled Absorption, Fluorescence, and Coherent Anti-Stokes Raman Spectrometry for Liquid Chromatographic Detection

Authors: Boutilier, G.D.1; Irwin, R.M.2; Antcliff, R.R.3; Rogers, L.B.3; Carreira, L.A.3; Azarraga, Leo4

Source: Applied Spectroscopy, Volume 35, Issue 6, Pages 525-611 (November/December 1981) , pp. 576-581(6)

Publisher: Society for Applied Spectroscopy

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Abstract:

A computer-controlled liquid chromatograph is described using two pumps for gradient elution. An absorption, fluorescence, and fluorescence-excitation detection system is centered around a silicon intensified target Vidicon detector. This allows fluorescence and absorption spectra to be recorded and stored every 3 s without stopping flow. A coherent anti-Stokes Raman spectrometer is also used in series in the detection scheme. This overall system allows great flexibility and the acquisition of a large amount of data from a single separation.

Keywords: Absorption spectra; Fluorescence spectra; Raman spectra; Vidicon spectra

Document Type: Research article

DOI: http://dx.doi.org/10.1366/0003702814732139

Affiliations: 1: Department of Chemistry, University of Georgia, Athens, Georgia 30602; present address: Proctor & Gamble Company, 6100 Center Hill Road, Cincinnati, OH 45224 2: Department of Chemistry, University of Georgia, Athens, Georgia 30602; present address: Arco Chemical, 500 South Ridgeway Ave, Glenolden, PA 19036 3: Department of Chemistry, University of Georgia, Athens, Georgia 30602 4: U.S. Environmental Protection Agency, Athens, Georgia 30602

Publication date: 1981-11-01

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