Computer-controlled Absorption, Fluorescence, and Coherent Anti-Stokes Raman Spectrometry for Liquid Chromatographic Detection

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Abstract:

A computer-controlled liquid chromatograph is described using two pumps for gradient elution. An absorption, fluorescence, and fluorescence-excitation detection system is centered around a silicon intensified target Vidicon detector. This allows fluorescence and absorption spectra to be recorded and stored every 3 s without stopping flow. A coherent anti-Stokes Raman spectrometer is also used in series in the detection scheme. This overall system allows great flexibility and the acquisition of a large amount of data from a single separation.

Keywords: Absorption spectra; Fluorescence spectra; Raman spectra; Vidicon spectra

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702814732139

Affiliations: 1: Department of Chemistry, University of Georgia, Athens, Georgia 30602; present address: Proctor & Gamble Company, 6100 Center Hill Road, Cincinnati, OH 45224 2: Department of Chemistry, University of Georgia, Athens, Georgia 30602; present address: Arco Chemical, 500 South Ridgeway Ave, Glenolden, PA 19036 3: Department of Chemistry, University of Georgia, Athens, Georgia 30602 4: U.S. Environmental Protection Agency, Athens, Georgia 30602

Publication date: November 1, 1981

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