Use of Thermal Blooming Spectroscopy for Enzymatic Analyses

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Abstract:

Thermal blooming has been used to measure weak absorption. The thermal lens was generated with a chopper ion laser (488 nm) and monitored with a helium-neon laser, using lock-in detection. The generation of dopachrome by enzyme-catalyzed air oxidation of dopamine was monitored as the rise of the dopachrome absorption signal. Dopamine concentrations were found to be proportional to initial slopes. Modifications to the existing apparatus to improve detection limits are suggested.

Keywords: Analysis, for dopamine; Methods, analytical; Thermal blooming

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702804731393

Affiliations: Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109

Publication date: July 1, 1980

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