A Simple and Versatile Microspectrophotometric System

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An easy-to-construct, cost-effective, and versatile microspectrometric system, employing a circular variable filter as a continuous wavelength scan monochromator, is presented. This simple system includes a single microscope optics, a single photodetector, and a spinning glass plate in conjunction with signal processing electronics, which allows a variety of microspectrometric operations at the cellular level. Three modes of operation, such as double-beam, double-beam derivative, and single-beam wavelength modulated derivative, are provided by minor rearrangements of the optics and the electronic modules. Some spectra of living cells are also presented to demonstrate the system performance.

Keywords: Microspectrophotometric system

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/0003702794926092

Affiliations: Department of Applied Physics, Osaka University, Suita, Osaka 565, Japan

Publication date: March 1, 1979

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