An easy-to-construct, cost-effective, and versatile microspectrometric system, employing a circular variable filter as a continuous wavelength scan monochromator, is presented. This simple system includes a single microscope optics, a single photodetector, and a spinning glass plate
in conjunction with signal processing electronics, which allows a variety of microspectrometric operations at the cellular level. Three modes of operation, such as double-beam, double-beam derivative, and single-beam wavelength modulated derivative, are provided by minor rearrangements of
the optics and the electronic modules. Some spectra of living cells are also presented to demonstrate the system performance.
Department of Applied Physics, Osaka University, Suita, Osaka 565, Japan
Publication date: March 1, 1979
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The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)