Authors: Chrisman, Ray W.; English, Jim C.; Tobias, R. Stuart

Source: Applied Spectroscopy, Volume 30, Issue 2, Pages 113-240 (March/April 1976) , pp. 168-179(12)

Publisher: Society for Applied Spectroscopy

Abstract:

The design and construction of a computer-controlled Raman spectrophotometer for the determination of difference spectra among up to four samples is described. The advantages of control by a minicomputer compared to a microcomputer or a hard wired controller are discussed. Conditions for the attainment of good signal/noise ratios with aqueous solutions of nucleotides in the 1 to 25 mM range are examined for the case of excitation in the visible spectrum to minimize radiation damage. Applications to studies of dilute aqueous solutions of biological molecules are discussed. The interactions of small molecules with biopolymers, particularly, are examined.

Keywords: Raman instrumentation; Difference spectroscopy; Biological molecules; Computerization

Document Type: Research article

DOI: http://dx.doi.org/10.1366/000370276774456327

Affiliations: 1: Department of Chemistry, Purdue University, West Lafayette, Indiana 47907

Publication date: 1976-03-01

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