Emission Spectrometric Determination of Trace Metals in Biological Tissues

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Abstract:

An emission spectrometric method of analysis is described which permits duplicate analyses to be performed on 1-g specimens of tissue for 13 elements in the microgram percent range. Pig hearts were dissected into 17 separate pieces representing discrete anatomic sites. Samples were prepared for analysis by low temperature wet digestion. On the basis of their composition of the macro elements sodium, potassium, and phosphorus, these tissues could be divided into two groups: one including blood vessels and heart valves, and the other including ordinary and specialized myocardium. A method is described for adjusting the macro element composition of these tissues to that of a common matrix, thus allowing trace metal analysis of both groups of tissues to be performed using only a single set of standards. A solution of the ash was placed in hollow cup graphite electrodes with center posts, evaporated to dryness, and analyzed in the de are with a Jarrell-Ash model 66,000 direct reading emission spectrometer. At concentrations above 10 μg%, the coefficient of variation was about 10% for most of the trace metals studied.

Keywords: Biological material; Cardiovascular tissues; Emission spectroscopy; Matrix effects; Methods, analytical; Trace metals

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370273774333281

Affiliations: 1: Division of Clinical Immunology and Rheumatology, University of Alabama School of Medicine, Birmingham, Alabama 35294 2: Division of Clinical Immunology and Rheumatology, University of Alabama School of Medicine, Birmingham Alabama 35294 3: Cardiovascular Research and Training Center, University of Alabama School of Medicine, Birmingham, Alabama 35294

Publication date: September 1, 1973

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