Formulas are derived which enable one to measure relative fluorescence quantum yields of strongly absorbing solutions. Relative fluorescence yield data are presented for quinine in N/10 H2SO4 at four different concentrations, demonstrating that the formulas are
correct to within ±3% for sample absorbances ranging from 1.36 to 0.017. The heart of these formulas, an expression giving the fraction of incident light absorbed behind the entrance slit of the emission monochromator, is presented in graphical form.
Bio-Medical Division, Lawrence Radiation Laboratory, University of California, Livermore, California 94550
Publication date: November 1, 1970
More about this publication?
The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)