Measurement of Relative Quantum Yields of Strongly Absorbing Solutions

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Formulas are derived which enable one to measure relative fluorescence quantum yields of strongly absorbing solutions. Relative fluorescence yield data are presented for quinine in N/10 H2SO4 at four different concentrations, demonstrating that the formulas are correct to within ±3% for sample absorbances ranging from 1.36 to 0.017. The heart of these formulas, an expression giving the fraction of incident light absorbed behind the entrance slit of the emission monochromator, is presented in graphical form.

Keywords: Absorbing solutions; Fluorescence; Quantum yields; Spectroscopic technique

Document Type: Research Article


Affiliations: Bio-Medical Division, Lawrence Radiation Laboratory, University of California, Livermore, California 94550

Publication date: November 1, 1970

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