If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

An Infrared Spectroscopic Study of the Hydrogen–Deuterium Exchange of Bovine Submaxillary Mucin

$29.00 plus tax (Refund Policy)

Buy Article:

Abstract:

An infrared spectroscopic method was used to study the hydrogen–deuterium exchange of a glycoprotein, bovine submaxillary mucin (BSM), dissolved in D2O. The pH-dependence of the rate and extent of the H–D exchange of BSM was determined. The rate constant of the exchange decreased as pH increased from 3.7 to 5.3 and remained constant at a minimum value, (0.82±0.09) × 10−2 min−1, from pH 5.3 to 7.2. The extent of the exchange decreased with increasing pH in the pH range 3.7 to 5.3 and levelled off from pH 5.3 to 7.2. It is suggested that these results are due to a pH-dependent conformational change.

Keywords: Glycoprotein; Hydrogen–Deuterium exchange

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370269774380905

Affiliations: Department of Biochemistry, New York Medical College, New York, New York 10029

Publication date: May 1, 1969

More about this publication?
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more