Application of Atomic Absorption in the Study of Na, K, Mg, and Ca Binding by Cellular Membranes

Authors: Sanui, Hisashi; Pace, Nello

Source: Applied Spectroscopy, Volume 20, Issue 3, Pages 135-194 (May/June 1966) , pp. 135-141(7)

Publisher: Society for Applied Spectroscopy

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Abstract:

Atomic-absorption spectrophotometry is applied in the analysis of biological membrane materials for micromolar levels of the physiologically important cations, Na, K, Mg, and Ca. Instrumental parameters and chemical interferences are investigated and a method for the measurement of all four cations in a single sample of ashed membrane material is formulated, tested, and applied. Results are highly satisfactory, and demonstrate that atomic-absorption spectrophotometry affords biologists a powerful and sensitive tool for the study of the important rôle played by inorganic ions in living systems.

Document Type: Research article

DOI: http://dx.doi.org/10.1366/000370266774386182

Affiliations: 1: Department of Physiology, University of California, Berkeley, California 94720

Publication date: 1966-05-01

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