Evaluation of Rotating Platform Technique for The Spectrochemical Analysis of Blood Cells

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A rotating platform technique is described for the spark analysis of ashed red blood cells with the direct reading spectrograph. A study was made of the effect of different variables that included the size and shape of the counter electrode, the density and surface configuration of the platform electrode, and the mode of sample application. It indicated that a 0.25 in. flat tip counter electrode, a flat platform electrode precoated with plicene, and one mg of sample residue to which a wetting agent had been added to facilitate sample application produced the best combination of sensitivity and reproducibility. The technique provided greater sensitivity than methods employing the rotating disc electrode, flat top electrode and the plastic cup electrode. Normal values are given for Ca, Mg, Cu, and Zn in red blood cells.

Document Type: Research Article

DOI: http://dx.doi.org/10.1366/000370264789620718

Affiliations: Department of Medicine, Queen's University, Kingston, Ontario, Canada

Publication date: March 1, 1964

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