About 20% of the sample required to fill a conventional one-cm ultraviolet spectrophotometer cell is actually traversed by the instrument beam. Clearly, then, only 20% of the sample present in the cell is absorbing energy and thus producing an instrument reading. Such inefficient utilization
of sample is ordinarily no disadvantage–the sample concentration is adjusted accordingly. However, with a limited amount of sample, it becomes increasingly important to achieve a high ratio of sample volume to cell volume.
The Ohio Oil Company, Denver Research Center, Littleton, Colorado
Publication date: February 1, 1962
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The Society publishes the internationally recognized, peer reviewed journal, Applied Spectroscopy, which is available both in print and online. Subscriptions are included with membership or can be purchased by institutional or corporate organizations. Abstracts may be viewed free of charge. Previously published as Bulletin (Society for Applied Spectroscopy)